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柴人杰
柴人杰
青年千人、东南大学生命科学研究院博导教授
领域:内耳干细胞调控机制的研究
  

个人简介

男,国家第十一批“青年千人计划”入选者,东南大学生命科学研究院教授。

个人经历

 2004年本科毕业于中国科学技术大学,生物科学专业。2009年博士毕业于美国贝勒(Baylor)大学,生物医学研究专业。2009年至2013年于斯坦福(Stanford)大学医学院从事博士后工作,博士后期间作为项目负责人主持的课题获美国斯坦福大学医学院院长奖励基金和美国听觉健康基金会资助,均已顺利结题。博士后期间两次获斯坦福大学医学院耳鼻喉系(第二届和第三届)研究成果讨论会优秀研究成果奖,两次获美国耳鼻喉研究学会(第34届和第35届)年会博士后旅行奖金。2013年8月开始在东南大学生命科学研究院任教授,博士生导师,同年10月获中华医学会第13次全国耳鼻咽学术会议优秀壁报奖, 2014年获国际内耳研究学会第57届年会优秀青年研究者旅行奖金。在东南大学期间先后获中组部青年千人计划,江苏省杰青,江苏省双创人才,科技部青年973计划,国家自然科学基金委面上项目,人社部留学人员科技活动重点项目,教育部留学人员项目,国家自然科学基金委青年项目,江苏省自然科学基金委青年项目,江苏省六大人才高峰高层次人才选拔培养计划,东南大学优秀青年教师教学科研资助计划,东南大学优青培育计划等项目的资助。

研究方向

内耳干细胞调控机制的研究

听力障碍是一种常见的耳疾。根据WHO 1985年全球听力残疾人数为4200万,1995年估计为1.2亿,2001年上升到2.5亿,2005年达到2.78亿,占全球人口的4.6%,其中80%残疾人群生活在中低收入国家。其中老年性耳聋的主要原因是由于内耳毛细胞和螺旋神经元的损伤,而永久性感音神经性聋主要是由于内耳毛细胞的不可逆损伤和由于毛细胞损伤而导致的螺旋神经元神经突的退化造成的。因此如何使内耳毛细胞和螺旋神经元神经突在损伤和丢失后修复和再生,是近年来听觉领域研究的重点。本实验室的研究方向主要着眼于Wnt信号通路对内耳干细胞的调控。通过对于内耳干细胞调控机制的研究,诱导这些干细胞增殖分化成为具有功能的耳蜗毛细胞和螺旋神经元细胞,从而治疗听力障碍。

学术成果

 近5年来发表SCI论文25篇,其中第一作者6篇,通讯作者2篇,另有国际会议大会发言2篇,国际会议论文22篇。

近期代表性论文:

X. Wang#, X. Wang#, Yuan W, R.J. Chai*(并列通讯作者), D. Liu*. Egfl6 is involved in zebrafish notochord development. Fish Physiol Biochem. 2015 May 8,doi: 10.1007/s10695-015-0061-x

T. Wang#, R.J. Chai#(并列第一作者),G. Kim, .X. Pham, L. Jansson, D. Nguyen, B. Kuo, L. May, E. Rubel, J. Zuo, L. Cunningham, A.G. Cheng*. Damage-Recruited Lgr5+ Cells Regenerate Hair Cells via Proliferation and Direct Transdifferentiationin Neonatal Mouse Utricle. Nature Communications, 2015 Apr 7;6:6613. doi: 10.1038/ncomms7613.

S. Sun, M. Sun, Y. Zhang, C. Cheng, M. Waqas, H. Yu, Y. He, B. Xu, S. Yin, R.J. Chai*(并列通讯作者), H. Li*. In vivo overexpression of X-linked inhibitor of apoptosis protein protects against neomycin-induced hair cell loss in the apical turn of the cochlea during the ototoxic-sensitive period. <a href=#, R.J. Chai#(并列第一作者), A. Lenoir, Z. Liu, L. Zhang, K. Steigelman, J. Fang, R. Palmiter, E. Rubel, A.G. Cheng*, J. Zuo*. Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development, 141(4):816-29. doi:10.1242/dev.103036. 2014

T.A. Jan#, R.J. Chai#(并列第一作者), Z. Sayyid, R. van Amerongen, A. Xia, T. Wang T, S.T. Sinkkonen, J.R. Levin, Y. Zheng, S. Heller, R. Nusse*, A.G. Cheng*. Tympanic border cells are Wnt-responsive and act as progenitors for postnatal mouse cochlear cells. Development 140, 1196-1206, doi:10.1242/dev.087528. 2013

R.J. Chai, B. Kuo, T. Wang, E. J. Liaw, A. Xia, T. A. Jan, Z. Liu, M. M. Taketod, J. S. Oghalaia, R. Nussec, J. Zuo, and A. G. Cheng*.Wnt signaling induces proliferation of sensory precursors in the postnatal mouse cochlea.Proc Natl Acad Sci U S A. 109(21):8167-72. doi: 10.1073/pnas.1202774109. 2012 May 22

R.J. Chai, A. Xia, T. Jan, T. Hayashi, O. Bermingham-McDonogh, A.G. Cheng*. Dynamic expression of Lgr5, a Wnt target gene, in the developing and mature mouse inner ear. Journal of the Association for Research in Otolaryngology (JARO); 12(4):455-69. PMCID: PMC3123443. 2011 Aug

R.J. Chai, S.Y Chen, J.H. Ding*, P. Grayburn*. Efficient, Glucose Responsive, and Islet-Specific Transgene Expression by a Modified Rat Insulin Promoter. Gene Therapy 16, 1202–1209 PMCID: PMC2762485. 2009

Y. He , D. Tang , C. Cai , R.J. Chai, H. Li*. LSD1 is Required for Hair Cell Regeneration in Zebrafish.Molecular Neurobiology 2015 May 26. [Epub ahead of print]

H. Mei, S.Sun, Y.Bai, Y. Y. Chen , R.J. Chai, H. Li*. Reduced mtDNA copy number increases the sensitivity of tumor cells to chemotherapeutic drugs.Cell Death & Disease2015 Apr 2;6:e1710. doi: 10.1038/cddis.2015.78.

R. Feng, Q. Sang, Y.Zhu, W. Fu, M. Liu, Y. Xu, H. Shi, Y.Xu, R. Qu, R.J. Chai,R. Shao, L.Jin, L. He, X.Sun, L. Wang*.MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro. Scientific Report 2015 Mar 3;5:8689. doi: 10.1038/srep08689

W. Li, J. Wu, J. Yang, S. Sun, R.J. Chai, Z. Chen* , H. Li*Notch inhibition inducesmitoticallygenerated hair cells in mammalian cochleae via activating the Wnt pathway. Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):166-71. doi: 10.1073/pnas.1415901112

Y. Chen, L. Li, W. Ni, Y. Zhang, S. Sun, D. Miao, R.J. Chai, H. Li*Bmi1 regulates auditory hair cell survival by maintaining redox balance.Cell Death & Disease 2015 Jan 22;6:e1605. doi: 10.1038/cddis.2014.549.

S. Sun, H. Yu, H. Mei, W. Ni, Y. Zhang, L. Guo, Y. He, Z.Xue, Y.Ni, J. Li, Y. Feng, Y.Chen, R. Shao, R.J. Chai,H. Li*.Inhibition of the Activation and Recruitment ofMicroglia-Like Cells Protects AgainstNeomycin-Induced Ototoxicity.Molecular Neurobiology 2015,51(1):252-267PMID: 24781382

D. Chang, R.J. Chai, R. DiMarco, S. Heilshorn, A.G. Cheng*. Protein-Engineered Hydrogel Encapsulation for 3D Culture of Murine Cochlea. Otology & Neurotology, 2015,36(3):531-538

Q. Sang, J. Zhang, R. Feng, X. Wang ,Q.Li ,X.Zhao,Q.Xing,Q.Chen, J. Du,S.Sun, R.J. Chai,, L. Jin, L. He ,H.Li* ,L.Wang*. Ildr1b is essential for semicircular canal development, migration of the posterior lateral line primordium, and hearing ability in zebrafish: Implications for a role in the recessive hearing impairment DFNB42.Human Molecular Genetic.2014 Dec 1;23(23):6201-11. doi: 10.1093/hmg/ddu340.

W. Yan , J. Li , R.J. Chai,W. Guo , L. Xu , Y. Han , X.Bai , H.Wang*.Combining Use of Captopril and Losartan Attenuates the Progress of Streptococcus pneumoniae-Induced Tympanosclerosis through the Suppression of TGF-β1 Expression.PLoS One. 2014 Oct 31;9(10):e111620. doi: 10.1371/journal.pone.0111620.

X. Bai, H. Lv, H. Zhang, J. Liu, Z.Fan, L.Xu, Y. Han, R.J. Chai,J. Li, H. Wang.Identification of a novel missense mutation in the WFS1 gene as a cause of autosomal dominant nonsyndromic sensorineural hearing loss in all-frequencies.Am J Med Genet A. 2014 Sep 23. doi: 10.1002/ajmg.a.36760. [Epub ahead of print]

Y. He, H. Yu, C. Cai, S. Sun, H, R.J. Chai,H. Li*Inhibition of H3K4me2 Demethylation Protects Auditory Hair Cells from Neomycin-Induced Apoptosis. Molecular Neurobiology 2014 Aug 19. 10.1007

X. Gu, L. Guo, H. Ji, S. Sun, R.J. Chai, L. Wang, H. Li*. Genetic testing for sporadic hearing loss using targeted massively parallel sequencing identifies 10 novel mutations. Clinical Genetics2014 May 22. doi: 10.1111/cge.12431

S. Volkenstein, K. Oshima, S. T. Sinkkonen, C. Corrales, S. Most, R.J. Chai, T. Jan, A.G. Cheng, S. Heller*. Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus. Proc Natl Acad Sci U S A. 2013 Aug 27, 110(35):14456-61. doi: 10.1073/pnas.1307376110.

A. Vu, J. Kim, M. Huth, L. Luk, R.J. Chai, A.J. Ricci, A. G. Cheng*. Integrity and regeneration of mechanotransduction machinery regulates aminoglycoside entry and sensory cell death Plos One, 2013, 8(1):e54794. PMCID:PMC3554584.

S. T. Sinkkonen, R.J. Chai, T. Jan, R. D. Laske, A.G. Cheng, K. Oshima, S. Heller*.Intrinsic regenerative potential of murine cochlear supporting cells Scientific Report2011 1, 26; DOI:10.1038/srep00026.

T. Jan, R.J. Chai, Z. Sayyid, A.G. Cheng*. Isolating LacZ-expressing cells from mouse inner ear tissues using flow cytometry. J Vis Exp, (58). 2011 Dec 23;