Dr. Evgeny Puchkov currently holds the positions of Leading Researcher at the Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino, Moscow Region) and Head of the Microbial Preservation Group at All-Russian Collection of Microorganisms which is a department at the Institute (1990-present). Also, he is Lecturing Professor at the State Institute of Natural Sciences (Pushchino, Moscow Region) for Master Degree students (1993-present). Previously, he was a Research Fellow, Senior Research Fellow and Head of Laboratory at the Institute of Applied Microbiology (Obolensk, Moscow Region) (1976-1990). He received a Master and a Candidate of Sciences (equivalent to PhD) degrees in Biophysics from the 2-Medical Institute (now University) of Moscow (1972 and 1976, respectively) and a Doctor of Sciences degree (the highest Scientific Degree in Russia) from the Institute of Applied Microbiology (1989). He is expert in various spectroscopic, microscopic and microbiological methods including long-term maintenance of live microbial cultures. His main scientific achievements are associated with the development of new approaches for both fundamental and applied research in microbiology. The methods he developed were successfully used for investigating intracellular physical-chemical parameters, functional features of biological membranes, cell viability assessment, mechanisms of damaging effects of low temperature and some antimicrobials on microorganisms, to mention a few. For the last decade, he is most interested in combining computer image analysis with optical methods for retrieving new information from microbiological objects. He has published more than 150 papers in journals (including several popular science papers), a book chapter, Author Certificates of Inventions and conference proceedings both in Russian and in English. He serves as a reviewer in international journals World Journal of Microbiology and Biotechnology, Acta Nature, Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology.